Crystal Violet

Crystal Violet structural formula

CAS Number548-62-9
Molecular FormulaC25H30ClN3
Molecular Weight407.990
InChI KeyZXJXZNDDNMQXFV-UHFFFAOYSA-M
LogP0.96
Synonyms
  • Gentian Violet
  • 4-{Bis[4-(dimethylamino)phenyl]methylidene}-N,N-dimethylcyclohexa-2,5-dien-1-iminium chloride
  • Methanaminium, N-[4-[bis[4-(dimethylamino)phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, chloride (1:1)
  • 548-62-9
  • Hexamethyl-p-rosaniline chloride
  • Methanaminium, N-[4-[bis[4-(dimethylamino)phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, chloride
  • Crystal violet
  • C.I. 42555
  • [4-[4,4'-Bis(dimethylamino)benzhydryliden]cyclohexa-2,5-dien-1-yliden]dimethylammoniumchlorid
  • [4-[4,4'-bis(dimethylamino)benzhydrylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride
  • 12416 Violet
  • Adergon
  • Aizen Crystal Violet
  • Aniline violet
  • Aniline violet pyoktanine
  • Atmonil
  • Avermin
  • Basic Violet 3
  • Basic Violet BN
  • Basonyl Violet 610
  • C.I. basic violet 003
  • C.I. Basic Violet 3
  • Calcozine Violet 6BN
  • Calcozine Violet C
  • Chlorure de [4-[4,4'-bis(dimethylamino)benzhydrylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium
  • chlorure de [4-[4,4'-bis(dimethylamino)benzhydrylidene]cyclohexa-2,5-diene-1-ylidene]dimethylammonium
  • cloruro de [4-[4,4'-bis(dimetilamino)bencidriliden]ciclohexa-2,5-dien-1-iliden]dimetilamonio
  • Crystal Violet 10B
  • Crystal Violet 5BO
  • Crystal Violet 6B
  • Crystal Violet 6BO
  • Crystal Violet AO
  • Crystal Violet AON
  • Crystal Violet BP
  • Crystal Violet BPC
  • Crystal Violet chloride
  • Crystal Violet FN
  • Crystal Violet HL 2
  • Crystal Violet Indicator
  • Crystal Violet O
  • Crystal Violet Pure DSC
  • Crystal Violet Pure DSC Brilliant
  • Crystal Violet SS
  • CRYSTAL VIOLET STAIN
  • Crystal Violet Technical
  • Crystal Violet USP
  • Gentersal
  • Gentiaverm
  • Genticid
  • Gentioletten
  • Hecto Violet R
  • Hectograph Violet SR
  • Hexamethyl violet
  • HEXAMETHYLPARAROSANILINE CHLORIDE
  • Hidaco Brilliant Crystal Violet
  • KRISTALLVIOLETT
  • Meroxyl
  • Meroxylan
  • Meroxylan-Wander
  • Meroxyl-Wander
  • Methanaminium, N-[4-[bis[4-(dimethylamino)phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, chloride (1:1)
  • Methyl Violet 10B
  • Methyl Violet 10BD
  • Methyl Violet 10BK
  • Methyl Violet 10BN
  • Methyl Violet 10BNS
  • Methyl Violet 10BO
  • Methyl Violet 5BNO
  • Methyl Violet 5BO
  • Methylrosaniline chloride
  • Methylrosanilinium chloride
  • Mitsui Crystal Violet
  • Oxiuran
  • Oxycolor
  • Oxyozyl
  • Pararosaniline, N,N,N',N',N'',N''-hexamethyl-, chloride
  • Plastoresin Violet 5BO
  • Pyoktanin
  • Sanyo Fanal Violet R
  • Vermicid
  • Violet CP
  • Violet XXIII
  • Aizen Crystal Violet Extra Pure
  • Caswell No. 264A
  • Crystal Violet chloride salt
  • Crystal Violet Extra Pure
  • Crystal Violet Extra Pure APN
  • Crystal Violet Extra Pure APNX
  • Crystal Violet HL2
  • EINECS 208-953-6
  • EPA Pesticide Chemical Code 039502
  • Hexamethyl p-rosaniline hydrochloride
  • Hidaco Crystal Violet
  • Methylrosanilini chloridum
  • Methylrosanilinum chloratum
  • NCI-C55969
  • NSC 3090
  • Paper Blue R
  • Pyoverm
  • Violet cristallise
  • Genapax
  • Chlorure de methylrosanilinum
  • Cloruro de metilrosanilina
  • Kristall-violett
  • Methylrosanilinchlorid
  • Methylrosanilinii chloridum
  • Metilrosanilinio cloruro
  • Violet gencianova
  • Violet krystalova
  • Violet zasadita 3
  • UNII-J4Z741D6O5
  • Hexamethyl para rosaniline chloride
  • (4-{bis[-(dimethylamino)phenyl]methylene}-2,5-cyclohexadien-1-ylidene)dimethylammonium chloride
  • Bismuth Violet
  • Blaues pyoktanin
  • Brilliant Violet 5B
  • C.I. Basic violet 3
  • CI 42555
  • CI Basic Violet 3
  • Gentianaviolett
  • N-(-4-{bis[4-(dimethylamino)phenyl]methylene}2,5-cyclohexadien-1-ylidene)-N-methyltrimethanaminium chloride
  • N-(4-{bis[4-(dimethylamino)phenyl]methylidene}cyclohexa-2,5-dien-1-ylidene)-N-methylmethanaminium
  • hexamethylpararosaniline chloride
  • 23355-47-7
  • 7077-31-8

Applications:

UV-Vis Spectrum of Crystal Violet

July 16, 2024
UV-Vis Spectrum of Crystal Violet. Absorption Maxima: 208 nm, 250 nm, 304 nm, 590 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Crystal Violet
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Michler’s ketone and Crystal Violet on Primesep 100 Column

August 18, 2023

High Performance Liquid Chromatography (HPLC) Method for Analysis of Michler’s ketone, Crystal Violet on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Michler’s ketone and Crystal Violet on Primesep 100 Column
HPLC Method for Analysis of Michler’s ketone, Crystal Violet on Primesep 100 Column by SIELC Technologies

Michler’s ketone (4,4′-bis(dimethylamino)benzophenone) is a chemical compound used primarily in the synthesis of dyes and pigments. It has the chemical formula C17H17N3O2. The compound is named after the German chemist Viktor Michler, who first prepared it.

Michler’s ketone is synthesized by condensing two equivalents of dimethylaniline with one equivalent of phosgene. The reaction involves the formation of a carbonyl group (C=O) that links two dimethylaniline moieties. The resulting structure features two aromatic rings (benzene rings) connected by a carbonyl group, with dimethylamino groups attached to each aromatic ring.

Michler’s ketone is a valuable intermediate in the dye industry, where it is used to prepare various triarylmethane dyes, including Malachite Green, Methyl Violet, and Crystal Violet. The strong electron-donating properties of the dimethylamino groups make it a useful compound for synthesizing dyes with vibrant and stable colors.

Michler’s ketone and Crystal Violet can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Michler’s ketone, Crystal Violet

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 80/20%
BufferGr H3PO4 – 0.2-0.4%, 10 min
Flow Rate1.0 ml/min
DetectionUV 371, Vis 590 nm
Peak Retention Time3.71, 8.57 min

Description

Class of CompoundsDyes
Analyzing CompoundsMichler’s ketone, Crystal Violet

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Crystal Violet
Michler’s ketone

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Crystal Violet on Primesep 100 Column

August 17, 2023

High Performance Liquid Chromatography (HPLC) Method for Analysis of Crystal Violet on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Crystal Violet on Primesep 100 Column
HPLC Method for Analysis of Crystal Violet on Primesep 100 Column by SIELC Technologies

Crystal Violet, also known as Methyl Violet 10B, is a synthetic dye belonging to the triarylmethane dye family. It has the molecular formula C25H30ClN3. This dye is primarily known for its use in microbiology as a stain, but it has other applications as well.

  1. Gram Staining: One of the most well-known uses of Crystal Violet is in Gram staining, a method used to differentiate bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties of their cell walls. In this process, a crystal violet solution is applied to a smear of bacterial cells, which are then treated with iodine, followed by an alcohol wash and counterstain with safranin. Gram-positive bacteria retain the crystal violet stain, while Gram-negative bacteria do not.
  2. Antiseptic: Crystal Violet has antiseptic properties and has been used as a topical antiseptic to treat minor burns, infections, and fungal conditions. However, due to concerns over toxicity, its use in this application has declined.
  3. Cell Biology and Histology: In cell biology and histology, Crystal Violet is used to stain cell nuclei, allowing for visualization under a microscope. It is also used in the assessment of cell viability and proliferation in various assays, such as the crystal violet cell cytotoxicity assay.
  4. Indicator in Chemistry: In analytical chemistry, Crystal Violet is used as an indicator in colorimetric titrations due to its ability to change color at different pH levels.
  5. Textile and Paper Industry: Crystal Violet is also used as a dye in the textile and paper industry.

Crystal Violet can be retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Crystal Violet

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 80/20%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionVis, 590 nm
Peak Retention Time9.37 min
Sample concentration0.0006 mg/ml
Injection volume5 µl
LOD10 ppb

Description

Class of CompoundsDyes
Analyzing CompoundsCrystal Violet

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Crystal Violet

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 Column

December 7, 2022

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 Column
HPLC Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 Column by SIELC Technologies

Pararosaniline (Basic Red 9) is a popular basic magenta dye and part of the triarylmethane family of dyes. Crystal Violet (Methyl Violet 10B), another basic triarylmethane dye, is frequently used for histological stains and for identifying Gram-positive bacteria. Crystal Violet Lactone, a derivative of Crystal Violet, is a basic thermochromic dye widely used as a security marker for various types of fuels. These three basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Pararosaniline, Crystal Violet and Crystal Violet Lactone

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 80/20%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV, 540, 590, 200 nm
Peak Retention Time3.15, 7.24, 8,82 min

Description

Class of CompoundsDyes
Analyzing CompoundsPararosaniline, Crystal Violet and Crystal Violet Lactone

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Crystal Violet
Crystal Violet Lactone
Pararosaniline Hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Dyes

November 21, 2010


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Condition 1

Column Newcrom B, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer H3PO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV, 275 nm

 


Various dyes are widely used in food, textile, and pharmaceutical industries. Dies are aromatic compounds containing ionizable groups. Methylene blue, crystal violet lactone, and crystal violet were separated on a Primesep D HPLC column. Method can be used for separation of other dyes and pigments that are basic and hydrophobic in nature.

Condition  2

Column Primesep D, 4.6×50 mm, 3 µm, 100A
Mobile Phase MeCN
Buffer H3PO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV, 300 nm

 

Description

Class of Compounds
Dye, Hydrophilic, Ionizable
Analyzing Compounds Methylene Blue, Crystal violet lactone, Crystal violet

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Crystal Violet
Crystal Violet Lactone
Methylene Blue

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.