HPLC Method for Analysis of Tylosin A, B, C, D on Primesep 100 Column

 

HPLC Method for Tylosin on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Tylosin

Tylosin A (C46H77NO17), Tylosin B (C39H65NO14), Tylosin C (C45H75NO17), and Tylosin D (C46H79NO17) are antibiotics that are typically used to treat infections and inflammations, especially in veterinary cases.

Tylosin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using ELSD.

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase Gradient MeCN/H2O from 10/90% to 60/40% 30 min
Buffer Ammonium Formate pH 3.0 – 60 mM
Flow Rate 1.0 ml/min
Detection UV 286

 

Class of Compounds Antibiotic
Analyzing Compounds Tylosin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Tylosin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.