Furan is a chemical contaminant that forms in some foods during traditional heat treatment techniques, such as cooking, jarring, and canning. The compounds can be retained and separated in HPLC using Newcrom R1 reverse-phase column. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 210 and 275 nm.
| Column | [mode] |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | 210, 275 nm |
| Class of Compounds | Hydrophilic, Acid, Aldehyde |
| Analyzing Compounds | 3,4-Bis(hydroxymethyl)furan, 5-Hydroxymethyl-2-furaldehyde (5-HMF), 5-(Hydroxymethyl)furan-2-carboxylic Acid, Furfuryl Alcohol, Furfural, 2-furancarboxylic acid |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
2-Furancarboxylic acid3,4-Bis(hydroxymethyl)furan
5-Hydroxymethyl-2-furaldehyde (5-HMF)
5-Hydroxymethyl-2-furancarboxylic acid
Furfural
Furfuryl alcohol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
