Rhein

Rhein structural formula

CAS Number478-43-3
Molecular FormulaC15H8O6
Molecular Weight284.223
InChI KeyFCDLCPWAQCPTKC-UHFFFAOYSA-N
LogP3.21
Synonyms
  • Rhein
  • 4,5-Dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-carboxylic acid
  • 2-Anthracenecarboxylic acid, 9,10-dihydro-4,5-dihydroxy-9,10-dioxo-
  • 478-43-3
  • 1,8-dihydroxy-3-carboxyl anthraquinone
  • 4-10-00-04088
  • 2-Anthroic acid, 9,10-dihydro-4,5-dihydroxy-9,10-dioxo-
  • BRN 2222155
  • Cassic acid
  • Chrysazin-3-carboxylic acid
  • 9,10-Dihydro-4,5-dihydroxy-9,10-dioxo-2-anthracenecarboxylic acid
  • 1,8-Dihydroxyanthraquinone-3-carboxylic acid
  • EINECS 207-521-4
  • Monorhein
  • NSC 38629
  • Rheic acid
  • Rhubarb Yellow
  • UNII-YM64C2P6UX

Applications:

Separation of Rhein on Newcrom R1 HPLC column

February 16, 2018
Separation of Rhein on Newcrom C18 HPLC column

Rhein can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Rhein
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.