Methylene Blue trihydrate

Methylene Blue trihydrate structural formula

CAS Number7220-79-3
Molecular FormulaC16H24ClN3O3S
Molecular Weight373.900
  • Methylene Blue trihydrate
  • 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride--water (1/1/3)
  • Phenothiazinium, 3,7-bis(dimethylamino)-, chloride, hydrate (1:1:3)
  • 7220-79-3
  • Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, trihydrate
  • 3,7-Bis(dimethylamino)phenazathionium chloride trihydrate
  • Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, hydrate (1:1:3)
  • 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride trihydrate
  • UNII-T42P99266K


Separation of Methylene Blue trihydrate on Newcrom R1 HPLC column

February 16, 2018
Separation of Methylene Blue trihydrate on Newcrom C18 HPLC column

Methylene Blue trihydrate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Methylene Blue trihydrate
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: or by phone: 847-229-2629.