| CAS Number | 6291-36-7 |
|---|---|
| Molecular Formula | C7H8ClNO3 |
| Molecular Weight | 189.601 |
| InChI Key | KKRSNTURHSOKTM-UHFFFAOYSA-N |
| LogP | 0.754 |
| Synonyms |
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Applications:
Separation of Mesalamine hydrochloride on Newcrom R1 HPLC column
May 16, 2018
Mesalamine hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsHPLC Separation of Isomers of Amino Salicylic Acid
November 21, 2010
| Column | Newcrom A, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
4-Aminosalicylic acid (PAS) is an antibiotic used in treatment of tuberculosis. It is a polar amino acid with limited retention on traditional C18 (reversed-phase) columns. Other isomers of aminosalicylic acid exist, but the main impurity in PAS is 5-Aminosalicylic acid, which also serves as anti-inflammatory drug. Both compounds are isomers with similar empirical structure and properties. These two isomers were separated on a Primesep 100 column with UV, ELSD and LC/MS compatible mobile phase. Method can be used a generic approach for separation of isomers of basic and zwitter ionic compounds. Isomers are retained and separated based on reversed-phase and cation-exchange properties. Retention time is controlled by the amount of acetonitrile, buffer concentration and buffer pH. Buffer pH is affecting ionization of these two compounds and thus serves as a powerful tool to adjust selectivity of separation.
| Column | Newcrom A, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0- 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 256 nm, MS-compatible mobile phase |
| Class of Compounds | Acids |
| Analyzing Compounds | 5-aminosalicylic acid, 4-aminosalicylic acid |
Application Column
Newcrom A
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options5-Aminosalicylic Acid (Mesalamine)
Mesalamine hydrochloride
