Pyridoxine, also known as Vitamin B6, is an essential nutrient required by the body to produce red blood cells and for proper nerve functioning. Doxylamine is a common treatment for insomnia. When taken together, they can treat morning sickness in pregnant women.
Pyridoxine and Doxylamine can be retained, separated, and detected on a normal-phase Primsep S using an isocratic analytical method with a simple mobile phase of water, Acetonitrile, and an Ammonium Formate buffer. This analysis method can be detected in the low UV regime at 270 nm.
| Column | Primesep S, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0- 100 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm, MS- compatible mobile phase |
| Class of Compounds | Hydrophobic, Drug |
| Analyzing Compounds | Pyridoxine, Doxylamine |
Application Column
Primesep S
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsVitamin B6 (Pyridoxine)
