Pyridoxine, also known as Vitamin B6, is an essential nutrient required by the body to produce red blood cells and for proper nerve functioning. Doxylamine is a common treatment for insomnia. When taken together, they can treat morning sickness in pregnant women.
Pyridoxine and Doxylamine can be retained, separated, and detected on a normal-phase Primsep S using an isocratic analytical method with a simple mobile phase of water, Acetonitrile, and an Ammonium Formate buffer. This analysis method can be detected in the low UV regime at 270 nm.
|Column||Primesep S, 4.6×150 mm, 5 µm, 100A|
|Buffer||AmFm pH 3.0- 100 mM|
|Flow Rate||1.0 ml/min|
|Detection||UV 270 nm, MS- compatible mobile phase|
|Class of Compounds||Hydrophobic, Drug|
|Analyzing Compounds||Pyridoxine, Doxylamine|
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.Select options
Vitamin B6 (Pyridoxine)