HPLC Method for Simultaneous Analysis of Piracetam and Levetiracetam in Pharmaceuticals

Separation type: Liquid Chromatography Mixed-mode

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Piracetam and Levetiracetam are two similar compounds in the racetam family with two varying degrees of effects on the body. Piracetam has been marketed as a treatment for myoclonus and as a way to enhance cognition, although neither claim is approved by the FDA. Levetiracetam, on the other hand, is a common treatment for epileptic seizures (and approved by the FDA).
These racetams can be detected in the low UV regime. There are two effective methods for retaining, separating, and analyzing these compounds. The first uses a SHARC 1 hydrogen-bonding column and a mobile phase consisting of acetonitrile (MeCN) and water without a buffer. The second uses Newcrom R1 mixed-mode column and a mobile phase consisting of acetonirile (MeCN) and water with a sulfuric acid (H2SO4) buffer. Either analysis method can be UV detected at 200 nm with high resolution.

Condition 1

Column SHARC 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O -98/2%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 200 nm

Condition 2

Column Newcrom R1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O -10/90%
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 200 nm



Class of Compounds
Drug, Racetam
Analyzing Compounds Piracetam, Levetiracetam


Do this now, take PEI smaller MW fraction and make a step method with 70% MeCN first for 2 min then 0% MeCN after. on ELSD as detector. 30 mM (NH4)2CO3
Ammonium bicarbonate 200 mM in H2O
7.9 g to 0.5 L H2O

Olga, I need to make this experiment. (NH4)2CO3 dissolved in water 100 mM. Then it mixed with MeCN 70%. having about 30 mM of the salt solution. Then you need to equlibrate AP column with this buffer. And inject diamino xylene

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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SIELC Technologies offers custom phases for customers who require specific separations not achievable with commercially available HPLC phases. Considering the vast array of compounds and mixtures requiring analysis, tailored LC phases can significantly enhance separation results for unique and challenging applications. To learn more about our special custom LC phases designed for your specific separation needs, please contact SIELC Technologies at research@sielc.com. Our team of experts is ready to guide you through the process and create a custom solution that addresses your particular chromatographic challenges.

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The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.