View on hplc.cloudp-Coumaric acid is the major phenolic acid in a wide variety of graminaceous plants. p-Coumaric acid is a natural metabolite contained in many edible plants and its antioxidant activities in reducing oxidative stress and inflammatory reactions have been demonstrated in various experiments The three isomers of coumaric acid can be detected at low UV. Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, the acids can be retained and UV detected at 270nm.
Newcrom BH, 4.6x150 mm, 5 µm, 100A
MeCN/H2O - 20/80%
H3PO4 - 1.0%
Class of Compounds
p -Coumaric Acid, .m -Coumaric Acid, o -Coumaric Acid