HPLC Separation of Coumaric acids on Newcrom BH Column

HPLC.cloud View on hplc.cloud p-Coumaric acid is the major phenolic acid in a wide variety of graminaceous plants.  p-Coumaric acid is a natural metabolite contained in many edible plants and its antioxidant activities in reducing oxidative stress and inflammatory reactions have been demonstrated in various experiments  The three isomers of coumaric acid can be detected at low UV.  Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, the acids can be retained and UV detected at 270nm.


Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H3PO4 - 1.0%
Flow Rate 1.0 ml/min
Detection 270 nm


Class of Compounds Acid
Analyzing Compounds p -Coumaric Acid, .m -Coumaric Acid, o -Coumaric Acid
Application Analytes:
m-Coumaric acid
o-coumaric acid
p-coumaric acid