HPLC Method for Analysis of Bupivacaine on Primesep B Column

HPLC Method for Analysis of Bupivacaine on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Bupivacaine on Primesep B Column by SIELC Technologies

Bupivacaine is a type of medication known as a local anesthetic. It works by blocking nerve impulses that send pain signals to your brain, numbing the area and reducing discomfort. This drug is most commonly used during surgical procedures, labor and delivery, or dental procedures. Bupivacaine is often injected into the area of the body where pain relief is needed.

Bupivacaine comes in various forms, including an injectable solution and a topical patch. It has a longer duration of action compared to some other local anesthetics, making it particularly useful for procedures that may require prolonged anesthesia or postoperative pain management.

As with any medication, bupivacaine can have side effects and potential risks. These can include low blood pressure (hypotension), irregular heart rhythms (arrhythmias), and in rare cases, severe reactions like seizures or cardiac arrest. Therefore, it’s crucial to only use bupivacaine under the direct supervision of a healthcare provider.

Bupivacaine can be analyzed on a reverse-phase Primesep B, 4.6 x 150 mm, 5 µm, 100 A column with a mobile phase consisting of water, Acetonitrile and Ammonium formate as a buffer modifier. This analysis method can be UV detected at 250 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Bupivacaine


ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN gradient
BufferAmFm pH 3.0
Flow Rate1.0 ml/min
DetectionUV 250 nm


Class of CompoundsAnesthetics, Amide
Analyzing CompoundsBupivacaine

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.