HPLC Method for Analysis of Bromoform on Newcrom R1 Column

HPLC Method for Analysis of Bromoform on Newcrom R1 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Bromoform on Newcrom R1 Column by SIELC Technologies

Bromoform is a heavy, volatile, colorless to yellow liquid, with a high refractive index, very high density, and sweet odor is similar to that of chloroform. It has the chemical formula CHBr3.

Bromoform is produced naturally in small quantities by algae and seaweed in the ocean, and it can also be synthesized by the haloform reaction of acetone (or ethanol) with sodium hypobromite, generated in situ by the reaction of sodium hydroxide with bromine.

In terms of uses, it was once used as a solvent, sedative and flame retardant, but now it is mainly used as a laboratory reagent.

One important thing to note is that bromoform is considered hazardous. It’s suspected of causing cancer, may cause damage to organs through prolonged or repeated exposure, and is harmful to aquatic life with long lasting effects.

Bromoform can be retained and analyzed on a reverse-phase Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A column with a mobile phase consisting of water and Acetonitrile (MeCN). This analytical method can detect compounds with high resolution and peak symmetry using UV detection at 220 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromoform


ColumnNewcrom R1, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O -70/30%
Flow Rate1.0 ml/min
DetectionUV 220 nm
Peak Retention Time3.52 min
Sample concentration0.57 mg/ml
Injection volume3 µl
Sample diluent MeCN/H2O -70/30%
LOD*0.01 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.


Class of CompoundsHaloforms
Analyzing CompoundsBromoform

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.