HPLC Method for Analysis of Propranolol on Primesep 100 Column

HPLC Method for Propranolol on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Propranolol

Propranolol is an organic compound with the molecular formula C16H21NO2.

Properties:
Appearance: Typically a white or off-white crystalline powder

Molecular weight: ~259.34 g/mol

Solubility: Soluble in water and ethanol

Uses: Beta-blocker medication for cardiovascular and non-cardiovascular conditions

Propranolol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 270 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 70%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 270 nm
LOD14 ppb

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsSynthetic Organic Compound
Analyzing CompoundsPropranolol

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Propranolol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.