HPLC Method for Analysis of Uric Acid on BIST B+ Column by SIELC Technologies

HPLC Method for Analysis of Uric acid on BIST B+ by SIELC Technologies.

Separation type: Hydrophilic interaction liquid chromatography (HILIC)

HPLC Method for Analysis of Uric acid on BIST B+ Column
HPLC Method for Analysis of Uric acid on BIST B+ by SIELC Technologies.

Uric acid is a waste product that’s produced when the body breaks down purines, substances found in foods and drinks like liver, anchovies, mackerel, dried beans, peas, and beer. It is normally excreted from the body in urine. However, if the body produces too much uric acid or doesn’t excrete enough of it, it can build up in the blood and potentially lead to health problems such as gout and kidney stones.

Uric acid can be retained and analyzed using an isocratic analytical method on a BIST B+ column. The simple mobile phase for this method comprises water, acetonitrile (MeCN), and formic acid as an ionic modifier. The analytical method can be monitored with UV detection at 260 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)


Column BIST B+, 4.6 x 150 mm, 10 µm, 100 A
Mobile PhaseMeCN 85%
BufferFA – 0.5%
Flow Rate1.0 ml/min
DetectionUV 290 nm
Peak Retention Time9.09 min


Class of CompoundsAcid
Analyzing CompoundsUric acid

Application Column


Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 10 µm
Pore Size: 100 A

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Application Analytes:
Uric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.