HPLC Method for Analysis of Polyhexanide (polyhexamethylene biguanide, PHMB) on BIST B+

HPLC Method forAnalysis of Polyhexanide (polyhexamethylene biguanide, PHMB) on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Analysis of Polyhexanide (polyhexamethylene biguanide, PHMB)
HPLC Method for Analysis of Polyhexanide (polyhexamethylene biguanide, PHMB) on BIST B+ by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Polyhexanide (polyhexamethylene biguanide, PHMB)

Polyhexanide (polyhexamethylene biguanide, PHMB) has multiple industrial, medical, biological and research applications. It has strong antiseptic properties that make this compound very useful for materials that could be a possible source of bacterial contamination.  Using SIELC’s newly introduced BIST™ method, Polyhexanide (polyhexamethylene biguanide, PHMB) can be retained on a positively-charged anion-exchange BIST™ B+ column. 

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Utilizing a step gradient to switch to a completely aqueous MP after 2 minutes allows for retention to occur while also preventing the method from being too long. Using this new and unique analysis method, PHMB can be separated, retained, and UV detected at 200 nm.


ColumnBIST B+, 4.6×50 mm, 5 µm, 100A
Mobile PhaseMeCN Step gradient
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Peak Retention Time3.3 min


Class of CompoundsPolymers
Analyzing CompoundsPolyhexanide (polyhexamethylene biguanide, PHMB)

Application Column


BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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Application Analytes:
Polyhexanide PHMB
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.