HPLC – MS Method for Analysis of Diquat on Obelisc R Column

HPLC Method for Analysis of Diquat on Obelisc R by SIELC Technologies

HPLC Method for Analysis of Diquat on Obelisc R Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Diquat

Diquat is a non-selective contact herbicide used in agriculture and landscaping. It’s known for its effectiveness in controlling a wide range of weeds and plants.

diquat is a potent herbicide used for controlling a broad spectrum of weeds and as a desiccant in agriculture. While effective, its toxicity and environmental impact necessitate careful handling and adherence to regulatory guidelines.

Diquat can be retained and analyzed on a Obelisc R mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%,
BufferAmmonium Formate pH 3.0-50 mM
Flow Rate0.2 ml/min
DetectionSIM 183 +, UV 310 nm
Spray Voltage:1.5 kV
Nebulizing gas:1.5 L/min
Drying gas:15 L/min
 DL temp:250 ˚C
Heat Block:400 ˚C

Class of Compounds
Analyzing CompoundsDiquat

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.