High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroquinone on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroquinone
Hydroquinone can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of Hydroquinone and information about its various lambda maxima by visiting the following link.
Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
Mobile Phase | MeCN – 1% |
Buffer | H2SO4 -0.05% |
Flow Rate | 1.0 ml/min |
Detection | UV 300 nm |
Samples | 1.0 mg/ml in MeCN/H2O – 50/50% |
Injection volume | 1 µl |
LOD* | 20 ppb (300 nm) |
Class of Compounds | Synthetic corticosteroid |
Analyzing Compounds | Hydroquinone |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A