HPLC Method for Analysis of Phenylephrine HCl on Primesep S Column by SIELC Technologies

HPLC Method for Analysis of Phenylephrine HCl on Primesep S Column

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Phenylephrine HCl on Primesep S Column by SIELC Technologies
HPLC Method for Analysis of Phenylephrine HCl on Primesep S Column by SIELC Technologies

Phenylephrine HCl is a fairly popular decongestant sold under many brand names including Mucinex, Sudafed PE, Sinex, and many other generic brands. While it is best known as a decongestant, it has a wide variety of other pharmaceutical applications, including pupil dilation, hypotension (low blood pressure) treatment, and hemorrhoid relief. Phenylephrine HCl can be detected in the low UV regime. Using a Primesep S normal-phase column and a mobile phase consisting of water and Acetonitrile (MeCN) with a Monosodium Phosphate (NaH2PO4) buffer, Phenylephrine HCl can be retained and analyzed. This analysis method can be UV detected at 220 nm.

ColumnPrimesep S, 4.6×100 mm, 3 µm, 100A
Mobile PhaseMeCN – 35%
Buffer

NaH2PO4 pH 4.5 – 2.6 g/l

Flow Rate2.0 ml/min
DetectionUV 220 nm
Retention Time4.12 min
Class of Compounds Drug
Analyzing CompoundsPhenylephrine

Application Column

Primesep S

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Phenylephrine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.