HPLC Method for Analysis of Chicago Sky Blue on BIST A+ Column by SIELC Technologies

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Chicago Sky Blue on BIST A+ Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analyses of Chicago Sky Blue

Chicago Sky Blue can be retained and separated into its component compounds easily on a negatively-charged, cation-exchange BIST™ A+ column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. While both of these sulfonated dyes have the same charge, their molecular structure (and therefore charge distribution) differs just enough to allow them to be separated in a high-organic MP. 

Other positively-charged buffers that can generate BIST™ include DMP, Calcium acetate, and Magnesium acetate. Using this new and unique analysis method, Brilliant Black BN and Chicago Sky Blue can be retained and separated with high selectivity and great peak shape. This method can be detected and is compatible with ELSD, CAD, and Mass Spectrometry (LC-MS).

Condition

ColumnBIST™ A+, 4.6×150 mm, 5µm, 100A
Mobile PhaseGradient MeCN
BufferTMDAP phosphate – 5 mM  pH 4.0
Flow Rate1.0 mL/min
DetectionVis 600 nm

Description

Class of CompoundsDyes
Analyzing CompoundsChicago Sky Blue

Application Column

BIST A+

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Chicago Sky Blue 6B

Application Detection:
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.