HPLC ELSD Method for Analysis Spermidine on Obelisc R Column

HPLC Method for Analysis of Spermidine on Obelisc R by SIELC Technologies

HPLC ELSD Method for Analysis Spermidine on Obelisc R Column
HPLC Method for Analysis of Spermidine on Obelisc R Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Spermidine

Spermidine is a polyamine compound that is found in all living cells. It is a biogenic amine and is involved in various cellular processes.

Biological Roles:

  • Cell Growth and Proliferation: Spermidine is involved in the regulation of cell growth and proliferation.
  • DNA Stability: It interacts with DNA and RNA, contributing to their stability.
  • Antioxidant Properties: Spermidine has been suggested to have antioxidant properties, potentially protecting cells from oxidative stress.
  • Autophagy Induction: Spermidine has been shown to induce autophagy, a cellular process that involves the degradation of cellular components.

Natural Sources:

  • Spermidine is found in various foods, including wheat germ, soybeans, mushrooms, and aged cheese.
  • It is also synthesized within the body from other polyamines.

Research and Health Implications:

  • Research on spermidine has gained attention for its potential health benefits, including its role in promoting longevity and protecting against age-related diseases.
  • Some studies suggest that spermidine supplementation may have positive effects on cardiovascular health, neuroprotection, and other aspects of aging.


  • Spermidine is available as a dietary supplement, and some people choose to take it for its potential health benefits.
  • However, it’s important to note that while research is ongoing, the long-term effects and optimal dosage for supplementation are not fully established.

Spermidine can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 20/80%
BufferAmmonium formate pH 3.0 – 30 mM
Flow Rate0.2 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 50°C,
 with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
Samples0.1 mg/ml
Injection volume5 µl
LOD*200 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Analyzing CompoundsSpermidine

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.