HPLC Method for Analysis of Spermidine on Obelisc R by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Spermidine
Spermidine is a polyamine compound that is found in all living cells. It is a biogenic amine and is involved in various cellular processes.
Biological Roles:
- Cell Growth and Proliferation: Spermidine is involved in the regulation of cell growth and proliferation.
- DNA Stability: It interacts with DNA and RNA, contributing to their stability.
- Antioxidant Properties: Spermidine has been suggested to have antioxidant properties, potentially protecting cells from oxidative stress.
- Autophagy Induction: Spermidine has been shown to induce autophagy, a cellular process that involves the degradation of cellular components.
Natural Sources:
- Spermidine is found in various foods, including wheat germ, soybeans, mushrooms, and aged cheese.
- It is also synthesized within the body from other polyamines.
Research and Health Implications:
- Research on spermidine has gained attention for its potential health benefits, including its role in promoting longevity and protecting against age-related diseases.
- Some studies suggest that spermidine supplementation may have positive effects on cardiovascular health, neuroprotection, and other aspects of aging.
Supplementation:
- Spermidine is available as a dietary supplement, and some people choose to take it for its potential health benefits.
- However, it’s important to note that while research is ongoing, the long-term effects and optimal dosage for supplementation are not fully established.
Spermidine can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD
| Column | Obelisc R, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Ammonium formate pH 3.0 – 30 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Samples | 0.1 mg/ml |
| Injection volume | 5 µl |
| LOD* | 200 ppb |
| Class of Compounds | Polyamines |
| Analyzing Compounds | Spermidine |
Application Column
Obelisc R
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
SpermidineApplication Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
