HPLC Method for Analysis of Prednisone on Primesep B Column

HPLC Method for Analysis of Prednisone on Primesep B by SIELC Technologies

HPLC Method for Analysis of Prednisone on Primesep B Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Prednisone

Prednisone is a synthetic corticosteroid drug that belongs to the class of glucocorticoids. It is a prodrug, meaning that it is biologically inactive until it undergoes metabolic activation in the liver, where it is converted into its active form, prednisolone. Both prednisone and prednisolone have anti-inflammatory and immunosuppressant properties and are used for various medical conditions.

  • Anti-inflammatory: Prednisone is used to reduce inflammation in various conditions such as arthritis, allergic reactions, and inflammatory bowel diseases.
  • Immunosuppression: It is employed to suppress the immune system in conditions like organ transplantation and certain autoimmune diseases.
  • Respiratory Conditions: Prednisone is often prescribed for respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD).

Prednisone can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 245 nm

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 35%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 245 nm

Class of Compounds
Analyzing CompoundsPrednisone

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
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