HPLC Method for Analysis of Prednisone on Primesep B Column

HPLC Method for Analysis of Prednisone on Primesep B by SIELC Technologies

HPLC Method for Analysis of Prednisone on Primesep B Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Prednisone

Prednisone is a synthetic corticosteroid drug that belongs to the class of glucocorticoids. It is a prodrug, meaning that it is biologically inactive until it undergoes metabolic activation in the liver, where it is converted into its active form, prednisolone. Both prednisone and prednisolone have anti-inflammatory and immunosuppressant properties and are used for various medical conditions.

  • Anti-inflammatory: Prednisone is used to reduce inflammation in various conditions such as arthritis, allergic reactions, and inflammatory bowel diseases.
  • Immunosuppression: It is employed to suppress the immune system in conditions like organ transplantation and certain autoimmune diseases.
  • Respiratory Conditions: Prednisone is often prescribed for respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD).

Prednisone can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 245 nm

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 35%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 245 nm

Class of Compounds
Corticosteroids
Analyzing CompoundsPrednisone

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Prednisone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.