HPLC Method for Analysis of Prednisolone on Newcrom CN2 Column

HPLC Method for Prednisolone on Newcrom CN2 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Prednisolone

Prednisolone is an organic compound with the molecular formula C21H28O5.

Properties:
Appearance: Typically a white to practically white, odorless, crystalline powder.

Molecular weight: ~360.4 g/mol

Solubility: Soluble in organic solvents like methanol, dioxane, and ethanol.

Uses: A widely used corticosteroid medication that provides relief from various health conditions by reducing inflammation and suppressing the immune system.

Prednisolone can be retained and analyzed using the Newcrom CN2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and phosphoric acid. Detection is performed using UV at 240 nm.

ColumnNewcrom CN2, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 10%
BufferH3PO4 – 0.1%
Flow Rate0.5 ml/min
DetectionUV 240 nm

Class of CompoundsCorticosteroid
Analyzing CompoundsPrednisolone

Application Column

Newcrom CN2

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Prednisolone
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.