HPLC Method For Analysis Of Minocycline hydrochloride on Newcrom R1 Column

Minocycline hydrochloride is a tetracycline antibiotic used to treat a wide variety of bacterial infections as well as treatment of acne vulgaris


Column Newcrom R1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer TEAPh – 20 mM, pH 2.0
Flow Rate 0.5 ml/min
Detection UV, 270 nm



Class of Compounds
Drug, Antibiotics
Analyzing Compounds Minocycline hydrochloride


Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Minocycline hydrochloride
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