HPLC Method for Analysis of Miconazole on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Miconazole
Miconazole is an antifungal medication with the chemical formula C18H14Cl4N2O. It’s most often used to treat skin and mucous membrane infections such as vaginal yeast infections, oral thrush, athlete’s foot, jock itch, and ringworm. Miconazole works by inhibiting the synthesis of ergosterol, an essential component of fungal cell membranes. This disruption leads to alterations in the membrane’s permeability and eventually results in fungal cell death.
Miconazole can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format a buffer. This analysis method can be detected in the UV 250 nm.
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 30-80%, 20 min |
| Buffer | Ammonium Formate pH 3.0-20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Allylamines |
| Analyzing Compounds | Miconazole |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
