HPLC Method for Determination of Miconazole in Health Care Products on Primesep B Column

HPLC Method for Analysis of Miconazole on Primesep B by SIELC Technologies

HPLC Method for Determination of Miconazole in Health Care Products on Primesep B Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Miconazole

Miconazole is an antifungal medication commonly used to treat fungal infections. Here are some key points about miconazole:

Usage: It’s most often used to treat skin and mucous membrane infections such as vaginal yeast infections, oral thrush, athlete’s foot, jock itch, and ringworm.

Mechanism of Action: Miconazole works by inhibiting the synthesis of ergosterol, an essential component of fungal cell membranes. This disruption leads to alterations in the membrane’s permeability and eventually results in fungal cell death.

Formulations: Miconazole is available in various formulations, including creams, powders, sprays, and suppositories.

Topical Cream: Applied to the skin to treat conditions like athlete’s foot, ringworm, and jock itch.
Vaginal Suppositories and Cream: Used for vaginal yeast infections.
Oral Gel: Used for oral thrush (a fungal infection in the mouth).

Miconazole can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format a buffer. This analysis method can be detected in the UV 250 nm.

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 30-80%, 20 min
BufferAmmonium Formate pH 3.0-20 mM
Flow Rate1.0 ml/min
DetectionUV, 250 nm
Class of Compounds
Analyzing CompoundsMiconazole

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.