Hydrochlorothiazide is a thiazide diuretic often considered the prototypical member of the class. It reduces the reabsorption of electrolytes from the renal tubules. This results in increased excretion of water and electrolytes, including sodium, potassium, chloride, and magnesium. It is used in the treatment of high blood pressure and swelling due to fluid build-up as well as several disorders such as edema, hypertension, diabetes insipidus, and hypoparathyroidism. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Hydrochlorothiazide. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Primesep 200, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 5-50% |
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Hydrochlorothiazide |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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