Separation type: Liquid Chromatography Reverse Phase
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethoxyquin
Ethoxyquin is an antioxidant. It was developed in the 1950s as a preservative to control the breakdown of fats in dry pet food or the formation of peroxide in canned pet foods. Ethoxyquin is also used as an insecticide, herbicide, fungicide, post-harvest dip to prevent scald on apples and pears, plant growth regulator, and anti-degradation agent for rubber. Ethoxyquin can be retained in HPLC on Newcrom R1 reverse-phase column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and Ammonium Formate (AmFm) buffer. Detection can be achieved with mass spectrometry (MS), evaporative light scattering detection (ELSD), charged aerosol detection (CAD) and UV detected at 275 nm.
| Column | Newcrom R1, 2.1×150 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | AmFm pH 3.0 – 10 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV, 275 nm |
| Class of Compounds |
Antioxidant, insecticide, herbicide, fungicide |
| Analyzing Compounds | Ethoxyquin |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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