HPLC Method for Analysis of Climbazole on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Climbazole
Climbazole is an antifungal agent commonly used in hair care products, primarily in shampoos, to treat dandruff and seborrheic dermatitis. It works by inhibiting the growth of the fungi that contribute to these conditions. Here are some key points about climbazole:
- Mechanism of Action:
- Climbazole is believed to inhibit the growth of fungi by interfering with ergosterol biosynthesis, which is a crucial component of fungal cell membranes. Without ergosterol, the fungal cell membrane becomes compromised, leading to the death of the fungus.
- Hair Care: The primary use of climbazole is in anti-dandruff shampoos and hair lotions. Dandruff and seborrheic dermatitis can be, in part, attributed to the overgrowth of the yeast Malassezia on the scalp. Climbazole helps control the proliferation of this yeast, thereby alleviating the symptoms associated with its overgrowth.
- Skincare: Occasionally, climbazole can also be found in skincare products designed for fungal-related skin conditions.
Climbazole can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format a buffer. This analysis method can be detected in the UV 270 nm.
|Column||Primesep B, 4.6 x 150 mm, 5 µm, 100 A|
|Mobile Phase||Gradient MeCN – 40-70%, 20 min|
|Buffer||Ammonium Formate pH 4.0-20 mM|
|Flow Rate||1.0 ml/min|
|Detection||UV, 275 nm|
|Class of Compounds||Allylamines|