HPLC Method for Determination of Climbazole in Dandruff Shampoo on Primesep B Column

HPLC Method for Analysis of Climbazole on Primesep B by SIELC Technologies

HPLC Method for Determination of Climbazole in Dandruff Shampoo on Primesep B Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Climbazole

Climbazole is an antifungal agent with the chemical formula C15H17ClN2O2. It is commonly used in hair care products, primarily in shampoos, to treat dandruff and seborrheic dermatitis. It works by inhibiting the growth of the fungi by interfering with ergosterol biosynthesis, which is a crucial component of fungal cell membranes. Without ergosterol, the fungal cell membrane becomes compromised, leading to the death of the fungus.

Climbazole can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format a buffer. This analysis method can be detected in the UV 270 nm.

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN – 40-70%, 20 min
BufferAmmonium Formate pH 4.0-20 mM
Flow Rate1.0 ml/min
DetectionUV, 275 nm
Class of Compounds
Allylamines
Analyzing CompoundsClimbazole

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Climbazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.