HPLC Method for Analysis of B-Estradiol on Primesep 200 Column

B-Estradiol is an estrogen steroid hormone. It is the major estrogen secreted by the premenopausal ovary. It is synthesized from testosterone primarily in the ovarian granulosa cells and placenta. B-estradiol is used as a medication, for instance in menopausal hormone therapy. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain B-Estradiol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

 

Condition

Column Primesep 200, 3.2×100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 5-50%, 5 min, 4 min hold
Buffer Gradient H2SO4 – 0.05-0.3 %, 5 min, 4 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Hormone, Hydrophobic
Analyzing Compounds B-Estradiol

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
B-Estradiol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.