HPLC Isocratic Method for Analysis of Ascorbic Acid and Sodium Metabisulfite on Primesep SB Column

HPLC Method for Analysis of Ascorbic Acid, Sodium metabisulfite on Primesep SB by SIELC Technologies

HPLC Isocratic Method for Analysis of Ascorbic Acid and Sodium Metabisulfite on Primesep SB Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Ascorbic Acid, Sodium metabisulfite

*The metabisulfite ion (S2O52−) is hydrolyzed to bisulfite (HSO3) in water. Sodium metabisulfite is a white crystalline or powder solid. It has many uses, but some of it’s more prominent are: as the source of SO2 in wine, as a bleaching agent in the production of Coconut cream, and added to anesthetic solutions to prevent oxidation to improve the shelf life of the solution. Ascorbic is found naturally in citrus fruits and many vegetables. As a medication, it is used to prevent or treat low levels of vitamin C, since it is that vitamin. Vitamin C is needed to maintain the health of skin, cartilage, teeth, bone, and blood vessels. Ascorbic Acid and Sodium Metabisulfite can be separated, retained, and analyzed on a Primesep SB mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a phosphoric acid (H3PO4) buffer. This analysis method can be detected in the UV 270 nm.

ColumnPrimesep SB, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 50/49.5%
BufferH3PO4 – 0.5%
Flow Rate1.0 ml/min
DetectionUV, 270 nm
Class of Compounds
Acid, Hydrophilic, Ionizable, Disinfectant, Antioxidant, Preservative agent.
Analyzing CompoundsAscorbic Acid, Sodium metabisulfite

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ascorbic Acid
Sodium metabisulfite

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.