HPLC Method for Allantoin on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Allantoin
Allantoin is found in plants such as wheat sprouts, chamomile, sugar beet, and comfrey. It is used as a moisturizer, which is, as the name suggest, used to moisturize the skin and to treat or prevent skin irritations and dry skin. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Allantoine. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
Column | Primesep 100, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O – 10/90% |
Buffer | TFA- 0.1% |
Flow Rate | 1.0ml/min |
Detection | UV, 210 nm |
Class of Compounds | Plant-based compound |
Analyzing Compounds | Allantoin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended