HPLC Method for Analysis of Oligonucleotides 10A on Chromni Column

HPLC Method for Oligonucleotides on Chromni™ by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Oligonucleotides

Oligonucleotide 10A is a modified oligonucleotide used in screening gene libraries as well as research using PCR, DNA sequencing, labeled probes, plasmid construction, and genomic manipulations.

Oligonucleotides can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.

ColumnChromni™, 2.1 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseGradient MeCN – 80-60%
BufferAmmonium Acetate
Flow Rate0.2 ml/min
DetectionUV 260 nm

Class of Compoundsnucleotides
Analyzing CompoundsOligonucleotides

Application Column

Chromni™

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Oligonucleotides
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.