HPLC Method for Analysis of Creatine and Creatinine on Chromni Column

HPLC Method for Creatine, Creatinine on Chromni™ by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Creatine, Creatinine

Creatine is a non-protein amino acid-like compound with the molecular formula C4H9N3O2.

Properties:
Appearance: Typically a white odorless powder.

Molecular weight: ~131.13 g/mol

Solubility: Soluble in water.

Uses: Dietary supplement.

Creatinine is a nitrogenous organic compound with the molecular formula C4H7N3O.

Properties:
Appearance: Typically white odorless crystals.

Molecular weight: ~113.12 g/mol

Solubility: Soluble in water.

Uses: Dietary supplement.

Creatine, Creatinine can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.

ColumnChromni™, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN – 70%
BufferPhosphoric Acid
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of Compoundsamino acid
Analyzing CompoundsCreatine, Creatinine

Application Column

Chromni™

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Creatine
Creatinine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.