HPLC Method for Analysis of Methyl cholate on Primesep B Column

HPLC Method for Analysis of Methyl cholate on Primesep B by SIELC Technologies

HPLC Method for Analysis of Methyl cholate on Primesep B Column
HPLC Method for Analysis of Methyl cholate on Primesep B Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Methyl cholate

Methyl cholate is a compound that belongs to the class of bile acids. Bile acids are substances that are produced by the liver and play a crucial role in the digestion and absorption of dietary fats. Cholate is a primary bile acid, and when methylated, it forms methyl cholate.

  • Methyl cholate is derived from cholic acid, one of the primary bile acids. The addition of a methyl group to cholic acid results in the formation of methyl cholate.
  • Bile acids, including cholic acid and its derivatives like methyl cholate, aid in the emulsification and digestion of fats in the small intestine. They also play a role in the absorption of fat-soluble vitamins.
  • Bile acids, including methyl cholate, undergo complex metabolic pathways in the liver and are released into the small intestine during digestion to aid in the breakdown of fats.

It’s important to note that bile acids have physiological roles beyond digestion, including the regulation of cholesterol metabolism and acting as signaling molecules.

Methyl cholate can be retained and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 192 nm

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 50/50%
BufferH3PO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 192 nm
Samples2 mg/mL in MeCN/H2O – 50/50%
Injection volume10 µl
LOD*0.2 ppm
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
bile acids
Analyzing CompoundsMethyl cholate

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Methyl cholate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.