HPLC ELSD Method for Analysis of Choline on Obelisc R Column

HPLC Method for Analysis of Choline on Obelisc R by SIELC Technologies

HPLC Method for Analysis of Choline on Obelisc R Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Choline

Choline is an essential nutrient that plays a crucial role in various physiological processes in the body. It is often grouped with B-vitamins, but it is not officially recognized as a vitamin. Choline serves several important functions, including:

  1. Cell Membrane Structure: Choline is a component of phospholipids, essential structural components of cell membranes. It helps maintain the structural integrity and fluidity of cell membranes.
  2. Neurotransmitter Synthesis: Choline is a precursor to acetylcholine, a neurotransmitter that is involved in muscle control, memory, and other cognitive functions. Adequate choline levels are important for maintaining proper neurotransmitter balance in the brain.
  3. Methyl Group Donor: Choline is a source of methyl groups, which are important for various biochemical reactions in the body, including the methylation of DNA, a process that regulates gene expression.
  4. Brain Development: Choline is particularly important during periods of rapid growth, such as fetal development and early childhood. Adequate choline intake during pregnancy is associated with better cognitive outcomes in children.
  5. Liver Function: Choline is involved in lipid metabolism and helps prevent the accumulation of fat in the liver. It is essential for the transport and metabolism of fats, and it plays a role in preventing non-alcoholic fatty liver disease.
  6. Muscle Function: Choline is involved in muscle movement and helps regulate the functioning of muscles.

Food Sources:
Choline is found in a variety of foods. Some good dietary sources of choline include:

  • Eggs
  • Meat, poultry, and fish
  • Dairy products
  • Nuts and seeds
  • Certain vegetables, such as broccoli and Brussels sprouts

Choline can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate as a buffer. Detection is achieved using ELSD

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 20/80%
BufferAmmonium acetate pH 5.0 – 10 mM
Flow Rate0.2 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 70°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
Samples1 mg/ml
Injection volume1 µl
LOD*200 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Quaternity amines
Analyzing CompoundsCholine

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.