HPLC ELSD Method for Analysis Butyrylcholine on Obelisc R Column

HPLC Method for Analysis of Butyrylcholine on Obelisc R by SIELC Technologies

HPLC ELSD Method for Analysis Butyrylcholine on Obelisc R Column
HPLC Method for Analysis of Butyrylcholine on Obelisc R Column by SIELC Technologies

Butyrylcholine is a choline-based ester that can function as a neurotransmitter with the chemical formula C9H20NO2+. It is a synthetic compound similar to acetylcholine. It is used primarily to distinguish between cholinesterases. You can find detailed UV spectra of Butyrylcholine and information about its various lambda maxima by visiting the following link.

Butyrylcholine can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 70/30%
BufferAmmonium formate pH 3.0 – 2 mM
Flow Rate0.2 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 50°C,
 with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
Samples1 mg/ml
Injection volume1 µl
LOD*60 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of Compounds
Quaternity amines
Analyzing CompoundsButyrylcholine

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Butyrylcholine
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.