HPLC Method for Analysis of Benzonatate on Primesep P by SIELC Technologies
Benzonatate is a medication with the chemical formula C30H53NO11. It is commonly used to relieve cough caused by conditions such as respiratory infections or irritation of the airways. It belongs to the class of drugs known as antitussives. Antitussives are substances that suppress or relieve coughing. Benzonatate works by anesthetizing the stretch receptors in the respiratory passages, lungs, and pleura. This helps reduce the urge to cough. It is typically taken orally in the form of capsules. It’s important not to chew or break the capsules, as this can lead to a numbing sensation in the mouth and throat. It is essential to follow the prescribed dosage and not exceed recommended limits. Overdose can lead to serious side effects, including death.
Benzonatate be retained and analyzed using a Primesep P mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and Ammonium formate as a buffer. This method allows for detection using UV at 310 nm
| Column | Primesep P, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 30-70%, 10 min, Gradient MeOH – 40-60%, 30 min |
| Buffer | Ammonium formate pH 3.0 – 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 310 nm |
| Class of Compounds | Acids, Aromatic carboxylic acids |
| Analyzing Compounds | Benzonatate |
Application Column
Primesep P
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
