HPLC Method for Analysis of Anthocyanins (Keracyanin, Delphinidin, Petunidin, Malvidin) on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Anthocyanins (Keracyanin, Delphinidin, Petunidin, Malvidin) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Keracyanin, Delphinidin, Petunidin, Malvidin on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Keracyanin, Delphinidin, Petunidin, Malvidin

Anthocyanins are a class of water-soluble pigments responsible for the red, purple, and blue colors found in many fruits, vegetables, and flowers. They belong to the flavonoid group of phytochemicals and have strong antioxidant properties.

Anthocyanins (Keracyanin, Delphinidin, Petunidin, Malvidin) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using Vis 530 nm.

You can find detailed UV-Vis spectra of Keracyanin, Delphinidin, Petunidin, Malvidin and information about its various lambda maxima by visiting the following links: Anthocyanins (Keracyanin, Delphinidin, Petunidin, Malvidin)

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionVis 530 nm

Class of Compounds
Anthocyanins
Analyzing CompoundsKeracyanin, Delphinidin, Petunidin, Malvidin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Delphinidin
Keracyanin
Malvidin
Petunidin
Application Detection:
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.