HPLC Determination of Taurolidine on Primesep Columns

 

Condition

Column Primesep S, Primesep 500 4.6×150 mm, 5 µm, 100Å
Mobile Phase MeCN – 100%
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

 

Description

Class of Compounds
Antibiotic, Non- charged polar molecule
Analyzing Compounds Taurolidine

 

 

 

 

 

 

Taurolidine is important medication used primarily as an antimicrobial agent in the prevention of bacterial infections in catheters. It has also been shown to have some anti-tumor properties.
Taurolidine is not approved for use in the United States, but widely used in the rest of the world.
HPLC analysis of taurolidine in reverse-phased mode poses a challenge with mobile phases that contain water. It undergoes hydrolysis when exposed to water and therefore requires pure organic mobile phase. We developed a simple method based on our mixed-mode column technology. Interesting that varying retention times, based on the molecular structure of ligand, can be achieved in pure bufferless acetonitrile (ACN) mobile phase using Sielc’s mixed-mode Primesep columns.

Application Column

Primesep S

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep 500

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Taurolidine

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.