HPLC Determination of Sunset yellow on Newcrom BH Column

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Sunset yellow (yellow 6) is a dye used in food, cosmetics and drugs. In the US, it has an acceptable daily intake of 3.75mg/kg. It can be retained on HPLC mixed-mode Newcrom BH column with a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) buffer. Vis detection at 480nm


Column Newcom BH, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 75/25%
Buffer H2SO4 - 0.5%
Flow Rate 1.0 ml/min
Detection Vis 480 nm


Class of Compounds Acid, Dyes
Analyzing Compounds Sunset yellow
Application Analytes:
Sunset Yellow (Yellow 6)