HPLC Method for Analysis of Sodium 3-chloro-2-hydroxypropane-1-sulfonate on Newcrom BH

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of sodium 3-chloro-2-hydroxypropane-1-sulfonate

 


Sodium 3-chloro-2-hydroxypropane-1-sulfonate is an anionic agent used to prepare negatively charged probe particles to investigate the nanoporosity of cellulosic fibers and their interactions with cationic polyelectrolytes.

Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, the constituent ions of sodium 3-chloro-2-hydroxypropane-1-sulfonate can be separated, retained, and analyzed. This analysis method can be detected by evaporative light scattering detection (ELSD). The ions can also be separated and retained using the same mobile phase, but with a sulfuric acid (H2SO4) buffer instead of the AmFm buffer. This method can be UV detected at 200 nm.

 

Condition 1

Column Newcrom BH, 3.2×100 mm, 3 µm, 100A
Mobile Phase MeOH/H2O – 10/90%
Buffer Ammonium Formate pH 3.0 – 40 mM
Flow Rate 0.5 ml/min
Detection ELSD

 

Condition 2

Column Newcrom BH, 3.2×100 mm, 3 µm, 100A
Mobile Phase MeOH/H2O – 10/90%
Buffer H2SO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV 200  nm

Description

Class of Compounds Sulfonate
Analyzing Compounds Sodium 3-chloro-2-hydroxypropane-1-sulfonate

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Sodium 3-chloro-2-hydroxypropane-1-sulfonate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.