HPLC Determination of Seleno Amino Acids on Newcrom AH Column


HPLC.cloud
View on hplc.cloud

Methylselenocysteine is used in chemotherapy to induce apoptosis (cell death) of the cancerous cells.  Methylselenocysteine is an amino acid with antioxidant properties and a main source of selenium in several foods.  Selenocysteine is an analog of cysteine with the selenium replacing sulfur.  It has been used as a radioactive tracer.  All three analytes are zwitterions and have similar structures which can present difficulties in reversed-phase separation in HPLC.  By using a mixed mode Newcrom AH column which has ion-exchange properties in addition to hydrophobic properties allows the separation of the seleno amino acids with an MS-compatible mobile phase of water and acetonitrile (ACN) with formic acid buffer.

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 20%
Buffer Formic Acid – 4%
Flow Rate 1.0 ml/min
Detection CAD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Methylselenocysteine, Selenomethionine, Seleno-L-cystine

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Methylselenocysteine
Seleno-L-cystine
Selenomethionine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.