HPLC ELSD Method for Analysis of Mogroside V on Primesep AP Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mogroside V  on Primesep AP by SIELC Technologies

HPLC Method for Analysis of Mogroside V  on Primesep AP by SIELC Technologies

Mogroside V is a natural compound and a type of cucurbitane glycoside with the chemical formula C60H102O29. It is primarily found in the fruit of the luo han guo (Siraitia grosvenorii) plant, also known as monk fruit. Monk fruit has been used in traditional Chinese medicine for its sweetening properties and potential health benefits.

Mogroside V  can be retained and analyzed on a Primesep AP mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and acetic acid as a buffer. This analysis method can be detected using ELSD

Condition

ColumnPrimesep AP, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 80%
Buffe Acetic acid – 0.5%
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 50°C,
with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
Sample0.7 mg/ml in MeCN/H2O – 50/50%
Injection volume5 µl

Description

Class of CompoundsBis-biguanide
Analyzing CompoundsMogroside V 

Application Column

Primesep AP

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Mogroside V 
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.