HPLC Determination of Folic Acid (Vitamin B9) on Newcrom BH Column

HPLC Method for Folic Acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Folic Acid.

Folic Acid, also known as vitamin B9, folic acid is crucial for many functions in the body, including DNA synthesis and repair, cell division, and growth. As an essential vitamin, Folic acid must be obtained through diet or supplementation, as humans cannot synthesize it. Vitamin B-9 is a crucial prenatal vitamin. that also helps the body use iron properly. It has the chemical formula C19H19N7O6. You can find detailed UV spectra of Folic Acid and information about its various lambda maxima by visiting the following link.

Folic acid can be retained in HPLC using a Newcrom BH mixed-mode column by using a mobile phase of acetonitrile (ACN) and water with Ammonium Formate (AmFm) as a buffer allowing the use of either UV or MS detector.

Condition
Column Newcrom BH, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 15/85%
Buffer AmFm – pH 3.0 – 20 mm
Flow Rate 1.0 ml/min
Detection UV 284 nm

 

Description
Class of Compounds
 Vitamin
Analyzing Compounds Folic Acid

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Folic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.