HPLC Determination of Folic Acid (Vitamin B9) on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode

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Folic acid can be retained in HPLC using a Newcrom BH mixed-mode column by using a mobile phase of acetonitrile (ACN) and water with Ammonium Formate (AmFm) as a buffer allowing the use of either UV or MS detector. Folic acid, vitamin B9, is an essential vitamin that’s used by the body to metabolise amino acids. Vitamin B-9 is a crucial prenatal vitamin. It supports cell growth, DNA synthesis, and red blood cell production. It also helps your body use iron properly.


Column Newcrom BH, 4.6x150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O - 15/85%
Buffer AmFm - pH 3.0 - 20 mm
Flow Rate 1.0 ml/min
Detection UV 284 nm


Class of Compounds Vitamin
Analyzing Compounds Folic acid, Vitamin B9
Application Analytes:
Folic Acid