HPLC Method for Analysis of Sodium erythorbate on Primesep B by SIELC Technologies
Sodium erythorbate is a common food preservative with the chemical formula C6H7NaO6. It has antioxidant properties, which makes it work well in a variety of meats, soft drinks, poultry, and occasional baked goods. Its use has increased as the US FDA has banned the use of sulfates in foods intended to be eaten fresh. It works through increasing the rate at which nitrite reduces to nitric oxide, which facilitates faster cure in meat. Sodium Erythorbate is derived primarily from beets, sugarcane, and corn.
Sodium erythorbate can be retained and analyzedon a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Ammonium acetate (AmAc) as the ionic modifier. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.
Condition
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 20% |
| Buffer | Ammonium Formate pH 3.0 – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Peak Retention Time | 6.81 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Sodium erythorbate |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
