HPLC Method for Analysis of Sodium   Erythorbate on Primesep B Column

HPLC Method for Analysis of Sodium erythorbate on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Sodium erythorbate on Primesep B Column by SIELC Technologies
HPLC Method for Analysis of Sodium erythorbate on Primesep B by SIELC Technologies

Sodium erythorbate is a common food preservative and curing agent with antioxidant properties found in a variety of meats, soft drinks, and baked goods. Sodium erythorbate can be retained and analyzedon a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Ammonium acetate (AmAc) as the ionic modifier. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium erythorbate

Condition

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 20%
BufferAmmonium Formate pH 3.0  – 30 mM
Flow Rate1.0 ml/min
DetectionUV 250 nm
Peak Retention Time6.81 min

Description

Class of CompoundsDrug
Analyzing CompoundsSodium erythorbate

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Sodium erythorbate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.