HPLC Method for Analysis of Sodium erythorbate on Primesep B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Sodium erythorbate is a common food preservative and curing agent with antioxidant properties found in a variety of meats, soft drinks, and baked goods. Sodium erythorbate can be retained and analyzedon a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Ammonium acetate (AmAc) as the ionic modifier. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium erythorbate
Condition
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 20% |
| Buffer | Ammonium Formate pH 3.0 – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Peak Retention Time | 6.81 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Sodium erythorbate |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
Sodium erythorbate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
