HPLC Determination of Diazepam on Newcrom R1 Column

Separation type: Liquid Chromatography Reverse Phase




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High Performance Liquid Chromatography (HPLC) Method for Analysis of DiazepamDiazepam (Valium) is a benzodiazepine.



It is thought that benzodiazepines work by enhancing the activity of certain neurotransmitters in the brain. Diazepam increases the activity of gamma-aminobutyric acid (GABA), a special chemical that can send signals throughout the nervous system. Diazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 240nm.



Condition
Column Newcrom R1, 2.1×100 mm, 5 µm, 100A
Mobile Phase MeCN – 30%
Buffer No
Flow Rate 0.2 ml/min
Detection 240 nm

Description
Class of Compounds Drug, Hydrophobic
Analyzing Compounds Diazepam

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Diazepam
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.