HPLC Determination of Darobactin on Primesep Columns

HPLC Method for Darobactin on Primesep 200 by SIELC Technologies

HPLC Determination of Darobactin on Primesep 200 Column_1212

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Darobactin 

The compound was discovered in Photorhabdus bacteria in 2019, living in the digestive systems of entomopathogenic nematodes. Researchers identified the nematode as a possible host because they feed on insects by targeting their larvae and releasing bacteria that then confront pathogens similar to those found in humans.

In experiments, it cured mice of Escherichia coli and Klebsiella pneumoniae infections, without toxic side effects.

ColumnPrimesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient  MeCN
BufferAmmonium Formate pH 3.0
Flow Rate1.0 ml/min
DetectionUV 280 nm
Class of CompoundsAntibiotic
Analyzing CompoundsDarobactin

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Darobactin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.