HPLC Method for Daptomycin on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis Daptomycin
Daptomycin is a cyclic lipopeptide antibiotic with the chemical formula C72H101N17O26. It displays a dose-dependent killing activity against a broad range of gram-positive bacteria. It has only minimal activity against anaerobic bacteria and no activity against gram-negative bacteria.
Daptomycin can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Daptomycin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
DaptomycinApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
