HPLC Method for Analysis of Butylated Hydroxytoluene on Newcrom R1 Column

HPLC Method for Analysis of Butylated hydroxytoluene on Newcrom R1 by SIELC Technologies

Separation type: Liquid Chromatography Reversed-phase

HPLC Method for Analysis of Butylated hydroxytoluene on Newcrom R1 Column by SIELC Technologies

Butylated hydroxytoluene (BHT) is a synthetic antioxidant widely used in a variety of industries, particularly in food preservation, cosmetics, and industrial products.

Butylated hydroxytoluene is used primarily for preserving the freshness and quality of products by preventing oxidative degradation. While considered safe for use within regulatory limits, its potential health and environmental effects continue to be areas of study and discussion.

Butylated Hydroxytoluene can be retained, and analyzed on a mixed-mode Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 280 nm using UV detection.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Butylated hydroxytoluene on Newcrom R1 Column by SIELC Technologies

Condition

ColumnNewcrom R1, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 80%,
BufferH2SO4 – 0.1%
Flow Rate0.5 ml/min
DetectionUV 280 nm
Injection volume2 µl
Sample1 mg/ml in MeCN/H2O – 80/20%
LOD*8 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsDiphenyl ethers
Analyzing CompoundsButylated hydroxytoluene

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Butylated hydroxytoluene
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.