Allantoin is a 5-ureidohydantoin or glyoxyldiureide with the chemical formula C4H6N4O3 and is found in plants like chamomile, wheat sprouts, tobacco seed, sugar beet and comfrey. Allantoin can be retained using HPLC on SHARC 1 column, which uses hydrogen-bonding as a separation mechanism with isocratic method by using a mobile phase of acetonitrile (ACN) and methanol (MeOH). Allantoin can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.
| Column | Sharc 1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/MeOH – 90/10% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds |
Hydrophilic, Amide, Amine |
| Analyzing Compounds | Allantoin |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
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