Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
HPLC Method for Analysis of Acid Red 1 on BIST A Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Acid Red 1
Acid Red 1 is a popular red synthetic dye used in a wide variety of food products. Using SIELC’s newly introduced BIST™ method, Acid Red 1, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged Acid Red 1 analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. The effect of reducing the solvation layer by increasing the organic component concentration in the mobile phase can be clearly seen above. Using this new and unique analysis method, Acid Red 1 can be retained and UV detected at 270 nm.
Condition
| Column | BIST™ A, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 80/20% |
| Buffer | TMDAP formate pH 4.0 – 5,0 mM |
| Flow Rate | 1.0 ml/min |
| Detection | Vis 535 nm |
Description
| Class of Compounds | Acid, Dye |
| Analyzing Compounds | Acid Red 1 |
Application Column
BIST A
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
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