HPLC Method for Analysis of 3-tert-butyl-4-hydroxyanisole (BHA) on Newcrom R1 Column by SIELC Technologies
3-tert-butyl-4-hydroxyanisole (BHA) is a synthetic antioxidant with the chemical formula C11H16O2. It is commonly used as a food preservative. It is an aromatic compound that belongs to a class of compounds known as butylated hydroxyanisoles. It is used to prevent the spoilage of fats and oils in various food products. It helps protect these substances from oxidative degradation, extending the shelf life of processed foods. The compound has a molecular structure with a tert-butyl group (tert-butyl radical) attached to the oxygen atom of a hydroxyanisole group. The IUPAC name for BHA is 2-tert-butyl-4-methoxyphenol. You can find detailed UV spectra of 3-tert-butyl-4-hydroxyanisole (BHA) and information about its various lambda maxima by visiting the following link.
3-tert-butyl-4-hydroxyanisole (BHA) be retained and analyzed on a reversed-phase Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 280 nm using UV detection
Condition
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 280 nm |
| Injection volume: | 1 µL |
| Sample | 1 mg/ml |
| Sample Diluent | MeCN/H2O – 50/50% |
| LOD* | 0.0135 ppm |
Description
| Class of Compounds | butylated hydroxyanisoles (BHAs) |
| Analyzing Compounds | 3-tert-butyl-4-hydroxyanisole (BHA) |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
